谢冬玲 王立东 李吉学 郭瑞峰 李艳杰 王启明 高珊珊 范宗民&nbs 2005-12-13 13:08:03 中华中西医杂志 2004年4月第5卷第8期
【摘要】 目的 探讨p15基因异常甲基化与贲门癌变的关系，探索其在临床早期基因诊断和治疗中的意义。方法 采用甲基化特异性聚合酶链式反应（MSP）法检测76例贲门癌患者癌组织与相应癌旁组织以及远癌正常胃粘膜的p15基因甲基化状态。结果 贲门癌患者癌组织p15基因启动子区甲基化阳性率为（18.1%），癌旁组织p15基因启动子区甲基化阳性率为（5.6%），而远癌正常胃粘膜均为非甲基化。结论 p15基因可通过甲基化而失活，可能与贲门癌的发生发展有关，其与p15基因表达的关系尚需进一步深入研究。关键词 贲门癌 p15基因甲基化 MSP
Analysis on the methylation of p15gene in gastric cardia cancer from the subjects at high-incidence area for gastric cardia cancer in henan Xie Dongling，
Wang Lidong，Li Jixue，et al.
Laboratory for Cancer Research，College of Medicine，Zhengzhou University，Zhengzhou450052.
【Abstract】 Objective To explore the relationship between the aberrrant methylation of the p15gene CpG isˉlands and gastric cardia cancer.Methods The level of the methylation of p15gene CpG islands in76cases of gastric cardiac cancer and matching adjacent non-cancer tissue and normal tissue were analysised by using methylation-specific PCR methods.Results p15gene CpG islands aberrant methylation were detected in18.1%gastric cardia cancer while were methylated in4cases（5.6%）adjacent non-cancer tissue and unmethylated in all normal issue.Conclusionp15gene can be inactived by CpG islands aberrant methyation，which perhaps actsan important role in the carcinogenesis of gastric cardia cancer.The relationship of methylation and p15gene expression remain to be furˉther studied.
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