人血T细胞在异种肾移植（猪→人）间接体内模型中的变化

　 来源：120online    　 日期：2007-05-04

　　摘　要　目的：探讨人血T细胞在异种肾移植（猪→人）间接体内模型中的变化。　方法：以模拟人体生理条件下的体外灌注系统，将人新鲜血灌注离体猪肾25只(实验组)，人肾12只(对照组)；利用流式细胞术，检测灌注前后不同时间内人血T细胞的变化。　结果：经猪肾灌注的人血T细胞在灌注一开始即锐减，由灌注前的67.8±3.4下降至32.4±2.1，下降了约52％；随着灌注时间的延长，其减少的幅度逐渐减弱。而对照组T细胞在灌注前后无明显变化。　结论：与同种移植不同，异种器官接通血流一开始，人血T细胞锐减，表明T细胞可能通过与猪肾血管内皮细胞的粘附，参与了异种移植排斥反应。

CHANGES OF T CELLS IN EX VIVO STUDY OF PIG-TO-MAN RENAL XENOTRANSPLANTATION

Wang Xi，Min Zhilian，Zhu Youhua，Qi Jun

　　Urological Center of PLA，Changzheng Hospital，Second Military Medical University of PLA，Shanghai 200003

　　OBJECTIVE To investigate the changes of T cell and in an ex vivo study of pig-to-man renal xenotransplantation．

　　METHODOLOGY Twenty-five pig kidneys and 12 human kidneys（as control）were perfused with human fresh blood in an ex vivo model of pig-to-man renal xenotransplantation．T cells labeled with immunofluorescent McAbs were analyzed by flow cytometer before and after perfusion．

　　RESULTS In the first minute of xenogenic perfusion，human T cells decreased 52％（from 67.8±3.4 to 32.4±2.1），and the magnitude of T cell decrease lessened there after．No significant change was observed in control group before and after perfusion．

　　CONCLUSION In this study，it is found that human T cells may play a role in the cell-mediated xenogeneic rejection of pig-to-man renal xenotransplantation possibly by adhering to procine renal endothelial cells during the perfusion．

　　Key words xenotransplantation kidney T cell

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