A Method of Preparing the Section of Eye Tissue for
Transmission Electron Microscopy
Tang Guo Tang Xiaojia Gan Dequan
Electron Microscopy Laboractory, West China University of Medical Sciences，Chengdu 610041
Abstract A method of preparing the section of eye tissue for transmission electron microscopy (TEM) is recommended. The tissue was prefixed with a mixed solution of 4% paraformaldehyde and 2.5% glutaraldehyde and followed by softening in 3% EDTA solution for 20 minutes, then the tissue was fixed in 1% osmium tetroxide, dehydrated in series acetone, infiltrated in Epox 812 for a longer, and embeded.Ultrathin sections were cut with glass knives or diamond knife, stained with uranyl acetate and lead citrate and examined with H600-Ⅳ. The advantage of this method is that the ultrastructures of tissues are well preserved without any damage and distortion. The main points of the procedure of preparation have been discussed.
Key words Eye tissue Transmission electron microscopy Preparation of specimen for TEM
1 前 言
眼组织结构复杂，眼球外形近似球体，外层巩膜为致密的结缔组织而内层又是较柔软的视网膜，易扭曲或剥离，角膜含大量纤维组织，眼球腔内有胶状玻璃体。根据眼球的特点，其制样难度极大，经过多年的探索，作者参考了国内外文献［1～4］，在制样过程中采取了相应的方法，获得了满意的结果，现报道如下：
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