Autodetection of Cell Adhesion by Light Transmission
Wang Jianmin Shi Yongde Guo Dehuang WuZhanqi Liang Zijun
Department of Biophysics, Shanghai Medical University, Shanghai 200032
Abstract To set up a new effective method for measuring the adhesion between red blood cells and vascular endothelial cells, we studied the relationship between adhesive red blood cells and light transmission by using Microscope-Photometric-Monolayer-Technique Elias-c Analyzer in our department. The results showed that the method of light transmission measurement on the adhesion between red blood cells and vascular endothelial cells is simple, effective and reliable.
Key words Cell adhesion Ligth transmission Computerized detection
1 前 言
血液的流变学特征，不仅包括红细胞的聚集性与变形性，而且涉及到红细胞与血管内皮细胞的相互作用、相互影响。因而近年来在血液流变学的研究领域中，有愈来愈多的研究者正致力于红细胞与血管内皮细胞粘附特征的研究［1］。红细胞与血管内皮细胞粘附性的异常变化，不仅反映了血液流变学特征的异常变化，而且是许多心血管病理生理变化的重要原因［2］。因此，简便、准确地检测红细胞与血管内皮细胞粘附也就十分重要。本室在德国亚琛大学赠送的细胞力学参数分析仪的基础上，建立了一种微机化光度法细胞粘附能力的检测方法。现介绍如下。
2 材料和方法
2.1 细胞力学参数分析仪的结构及细胞粘附的检测原理［3］
