Formation and Characteristics of an Artificial Dermis——
Collagen-Chondroitin-6-Sulfate Membrane
Li Xiaojian Shi Jixiang
Guangzhou Red Cross Hospital,Guangzhou 510220
Ruijing Hospital Attached to shanghai second Medical University,Shanghai 200025
Abstract In this study, we extracted type-I collagen from rat tail tendon with acetic acid and then mixed it with chondroitin-6-sulfate to form a collagen-chondroitin-6-sulfate membrane by freeze drying and crosslinking. The result showed that the collagen-chondroitin-6-sulfate membrane was semitransparent, with a network structure, and the pore size was about 100-150μm. It was also demonstrated that the “dermis” achieved a good adherence to wound and the degradation rate in vitro was markedly decreased by glutaraldehyde crosslinkage.
Key words Collagen Chondroitin-6-sulfate Glutaraldehyde
近十几年来，复合移植方法覆盖Ⅲ度烧伤切痂创面越来越受到人们的重视，其中理想的真皮成份甚为重要。我们参照Yannas［1］的方法以改进制成的人工真皮具有组织相容性好、创面粘附力强、不易被降解吸收等优点，而且内部为有适宜孔径大小的网状结构，能诱导创面新生真皮的再生。现将制作方法及部分生物物理性能介绍如下。
1 材料和方法
1.1 胶原+6-硫酸软骨素膜的制作
