几种胶原型创伤敷料制作的实验研究

　 来源：120online    　 日期：2007-05-09

　　摘要　介绍了几种胶原型创伤敷料研制的方法。冷冻牛腱经0.05 M乙酸处理(pH3.2)48～72 h后，捣碎、过滤、脱泡、加硫酸软骨素(8%)，制成1.5%～2.5%胶原溶液。该溶液在预冷或不预冷的模具内冷冻干燥，冻干的胶原海绵在0.25%戊二醛溶液中交联24 h。并以类似的方法研制成聚氨酯膜-胶原海绵复合膜，涂聚氨酯胶原膜，和纱布胶原膜三种创伤敷料。结果表明冷冻牛腱胶原性能稳定，冻干的胶原海绵具有良好的孔洞结构(50～400 μm)，但气面孔洞比模具面的孔洞大数倍。本文简要讨论了影响胶原海绵孔洞结构和大小的有关因素，以及他们作为创伤敷料的可行性。

Preparation of Collagen-based Materials for Wound Dressing

　　Wu Zhigu　Sheng Zhiyong　Sun Tongzhu　Geng Miao　Zhou Baotong

　　Li Junyou　Huang Zuxiu

　　Burns Institute，304th Hospital PLA,Beijing　100037

　　Department of Chemistry, Beijing University,Beijing　100871

　　Abstract　This article addressed the methods to develop collagen-based materials for wound dressing. Fresh frozen bovine tendon was treated with 0.05M acetic acid at pH3.2 for 48-72 h,then it was homogenized, filtered, and after the process of adding chondroitin sulphate(8%),it was debubblized; thus 1.5%-2.5% collagen solution was finally prepared. The solution was lyophilized in prefrozen or un-prefrozen mold. The collagen sponge was crosslinked with 0.25% glutaraldehyde for 24 h. Three other wound dressings were developed by similar method, namely collagen membrane with polyurethane membrane onlay, polyurethane-coated collagen membrane and collagen membrane on gauze. It was demonstrated that the property of frozen bovine tendon was stable, and the prepared collagen sponge contained pores of 50-400 μm in diameter. Some factors influencing pore structure and size in the collagen sponge were discussed. The results of this preliminary study suggested that the collagen could be used as wound dressing.

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