应用图象处理系统对深低温停循环后神经细胞中钙颗粒定量分析

　 来源：120online    　 日期：2007-05-07

　　摘　要　目的　用草酸－焦锑酸钾细胞化学法显示深低温（18℃）停循环（DHCA）120分钟后脑神经细胞内Ca²⁺的分布及超微结构改变。方法　利用图象处理系统同时对正常组、对照组及保护组细胞内钙颗粒的体密度、数密度及颗粒大小等进行定量分析。结果　DHCA120分钟后脑神经细胞超微结构破坏严重，细胞内钙颗粒明显增加。但在DHCA期间应用1,6－二磷酸果糖（FDP）脑保护液后，细胞内钙颗粒明显减少，超微结构破坏明显减轻。结论　在DHCA中,

　　fDP可以有效地防止脑细胞的损伤。

　　

　　中图号　R318.03;R338.25

　　Quantitative Study on Ca²⁺ granules in Neuron after Deep Hypothermic Circulatory Arrest by

　　using Image Processing System Li Li, Shao Xinli, Liu Chunxin, et al.Center of Analysis & Measurement,

　　wuhan University, Wuhan 430072

　　Abstract　Objective　The distribution of Ca²⁺ and ultrastructural changes in brain neuron after 120

　　minutes of deep hypothermic (18℃) circulatory arrest (DHCA) were determined by using K-pyroantimonate

　　cytochemistry method.Methods　The image processing system was employed to measure the volume density,

　　count density as well as the size of the intracellular Ca²⁺ granules of the normal group, contrasting group and

　　the protected group.Results　The ultrastructure of brain neuron was damaged severely, and the intracellular

　　ca²⁺ granules increased significantly after 120 minutes of DHCA. When fructose-1,6-diphosphate (FDP)

　　cerebro-protection liquid was employed during DHCA, the number of intracellular ca²⁺ granules decreased

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