放线共生放线杆菌白细胞毒素水平检测

　 来源：120online    　 日期：2007-05-07

　　【摘要】　目的　检测放线共生放线杆菌（Actinobacillus actinomycetemcomitans, Aa）临床分离菌株白细胞毒素水平，区分高毒株与低毒株。方法　应用聚合酶链反应（polymerase chain reaction, PCR）检测白细胞毒素操纵子启动子区域基因序列的差异。检测临床菌株68株，其中b型17株，c型42株，a型9株。阳性对照高毒株为JP2，低毒株为ATCC43717等5株Aa国际参考菌株，阴性对照为12株异种菌国际参考菌株。结果　68株临床分离菌株扩增片段均为1 022 bp, JP2扩增片段为492 bp，ATCC43717等5株扩增片段均为1 022 bp，12株异种菌参考菌株无扩增片段出现。结论　68株临床分离菌株全部为低毒株。

Detecting the level of Actinobacillus actinomycetemcomitans leukotoxin

WANG Zheling

　　（Research Institute of Stomatology，Beijing Stomatological Hospital，Beijing 100050，China）

　　ZHANG Tingfa ,XIAO Bai

　　【Abstract】　Objective　To detect the level of Actinobacillus actinomycetemcomitans (Aa) leukotoxin and distinguish the highly and the minimally toxic strains.Methods　The polymerase chain reaction (PCR) assay was used to detect the difference of the nucleotide sequence of Aa leukotoxic promoter. 68 Aa isolates were examined, including 17 serotype b,42 serotype c and 9 serotype a strains. The positive control strains were the highly toxic strain JP2 and 7 minimally toxic strains such as ATCC43717; the negative control strains were 12 other species of reference strains.Results　The PCR fragments of JP2 was about 492 bp and that of the other Aa reference strains and 68 clinical isolates were 1 022 bp. PCR products not found in 12 other species.Conclusion　All of the 68 clinical isolates were minimally toxic strains.

　　【Key words】　Actinobacillus；　Cytotoxins；　Periodontitis

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