4NQO诱发两种品系大鼠舌癌过程中p53蛋白的表达特点

　 来源：120online    　 日期：2007-05-07

　　【摘要】　目的　了解Dark-Agouti (DA)和Wistar/Furth (WF)两种品系的大鼠舌粘膜上皮在4-硝基喹啉-1-氧化物（4-nitroquinoline 1-oxide，4NQO）诱导舌癌过程中p53蛋白表达的动态变化。方法　实验动物的饮水中加入4NQO（0.001%），观察第2、4、8、12、16和26周时实验大鼠舌粘膜对p53的免疫组化表达。结果　p53的阳性反应在DA大鼠舌粘膜中出现早，且随着实验的推移，舌粘膜内的p53阳性细胞簇数目趋于增加，特别在实验后期尤为显著；WF大鼠各实验组间p53表达的水平较低，且变化不大。结论　p53蛋白的表达水平反应了DA和WF大鼠对4NQO诱发舌癌易感性的差异。

Expression of p53 protein during 4NQO-induced lingual carcinogenesis in two strains of rats

LI Tiejun.

　　( Department of Oral Pathology, School of Stomatology, Beijing Medical University, Beijing 100081，China)

　　【Abstract】　Objective　To investigate p53 protein expression during lingual carcinogenesis in two strains of rats.Methods　Dark-Agouti (DA) and Wistar/Furth (WF) rats were administrated with 4-nitroquinoline 1-oxide (4NQO) in drinking water and expression of p53 was studied using experimental materials obtained at 2, 4, 8, 12, 16, and 26 weeks of 4NQO treatment.Results　Epithelial clusters of p53 positive cells appeared earlier and more in numbers in DA rats than in WF. A marked increase of p53⁺ clusters in DA rats was noted at later stages of the experiment, whereas those in WF rats appeared to be unchanged.Conclusions　These results suggest that there may be genetically determined variations in the p53 response to 4NQO between DA and WF rats, and these differences may contribute to their contrasting susceptibility during lingual carcinogenesis.

　　【Key words】　Genes，p53；　Carcinogenicity tests；　Rats

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