聚合酶链反应与常规方法检测牙龈卟啉单胞菌的比较研究

　 来源：120online    　 日期：2007-05-07

　　摘　要：目的　使用聚合酶链反应 (polymerase chain reaction, PCR)检测龈下菌斑的牙龈卟啉菌，并与常规的培养法和间接免疫荧光法相比较。方法　设计纤毛亚单位蛋白基因内的一对引物，用PCR对92个龈下菌斑标本进行扩增，与其他两种方法的检出率进行比较。结果　相同的菌斑标本，PCR的检出率为94.6%，培养法为48.9% ，间接免疫荧光法为68.5% ，前者明显高于后两者(P＜0.001)。结论　对牙周病致病菌的检测，PCR较培养法和间接免疫荧光法具有较高的敏感性。本项研究建立了一种比较适合临床应用的牙周菌斑PCR检测方法。

A comparison of polymerase chain reaction with other two widely used methods in detection of Porphyromonas gingivalis

JIN Xiaoyi

　　（Beijing Stomatology Hospital , Capital University of Medical Science, Beijing 100050, China）

　　YANG Shenghui

　　WANG Shenwu

　　Abstract：Objective　To compare the efficiency of polymerase chain reaction (PCR) with cultural method and indirect immunofluorescence(IF) in detection of Porphyromonas gingivalis (P.g) in oral plaque samples.Methods 92 subgingival plaque samples were collected from patients of three kinds of periodontal disease. A program using PCR to detect Fimbrilin gene (Fim A) was designed to detect P.g. The efficiency of this PCR method was compared with cultural method and IF. Results The positive-rate of PCR was 94.6%, while that of cultural method and IF was only 48.9% and 68.5% respectively. The difference was significant in statistical analysis (P＜0.001).Conclusion PCR is more sensitive than culture method and IF in detecting pathogens of periodontal diseases. The study provides a simplified and efficient method for oral clinical use.

　　Key words：Polymerase chain reaction (PCR)；Porphyrins；Gene▲

[1] 2 3 4 5  下一页

关键字：

下一篇：菌斑pH值、游离钙和总蛋白水平与根龋易感性的关系 

上一篇：降钙素基因相关肽阳性神经纤维在鼠颞下颌关节中的分布 

返回首页　推荐给好友　收藏这篇文章