综上所述，本项研究所提出的PCR检测方法具有快速、简便、高效的特点，可望成为口腔科临床致病菌检测的新方法，值得进一步深入研究。
参考文献：
［1］Mayrand D, Holt SC. Biology of a saccharolytic black-pigmented bacteroides species. Microbiol Rev, 1988, 52:134-152.
［2］Watanabe K, Frommel TO. Detection of porphyromonas gingivalis in oral plaque samples by use of the polymerase chain reaction. J Dent Res, 1993, 72:1040-1044.
［3］杨圣辉，张春梅，任蕾，等.用间接免疫荧光快速检测牙周袋 内牙龈类杆菌. 中华微生态学杂志, 1990, 2: 31-33.
［4］金笑一，杨圣辉, 张春梅，等. 多聚酶链反应检测牙龈卟啉单胞菌. 北京口腔医学, 1997, 5: 58-60.
［5］Slots J, Listgarten MA. Bacteroides gingivalis, Bacteroides intermedius and Actinobacillus actinomycetemcomitans in human periodontal disease. J Clin Periodontol, 1988, 15: 85-93.
［6］Ashimoto A, Chen C, Bakker I, et al. Porphyromonas chain reaction detection of 8 putative periodontal pathogens in subgingival plaque of gingivitis and advanced periodontitis lesion. Oral Microbiol Immunol, 1996, 11:266-273.
