中图分类号:R73.3;R735.2 文献标识码:A
文章编号:1009-4571(2000)01-0026-02
The effect of newcastle disease virus on the human lines of gastric cancer
LI Xiao-jun YIN Zhi-liang TIAN Fu-zhou et al
(General Surgical Center of PLA,General Hospital of Chengdu Military Command,Chengdu 610083)
【Abstract】 Objective:To study the effect of newcastle disease virus (NDV) on the human lines of gastric cancer cells.Methods:The killing effect of NDV on gastric cancer cells was examined by MTT method.Results:The cell viability was higher in control group than that in experimental group (P<0.01)and the hemoglutination titer of NDV was higher in control group.Conclusions:NDV can kill tumor cells and can be used to treat tumor.
【Key words】 newcatle disease virus;gastric carcinoma
新城疫病毒(简称NDV)属副粘病毒Ⅰ型,具有抗肿瘤作用,并且已应用于肿瘤的研究[1,2]。本研究探讨了NDV对人胃癌细胞的抑制杀伤作用。
1 材料与方法
1.1 细胞株
人胃癌细胞株SGC-9701、BGC-823、MGC-823。采用RPM 1 640含15%小牛血清培养液进行培养,人胃癌细胞PAMC 82采用DMEM含10%小牛血清培养液培养,条件为37℃、5%CO2培养箱;新城疫病毒由四川省农业科学院引进,本中心接种于SPF-9d鸡胚,增殖后超速离心、收集、纯化病毒,经红血球凝集检测效价为1∶840。
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