【摘要】 目的 观察脑源性神经营养因子（BDNF）基因工程成肌细胞脑内纹状体移植对帕金森病（PD）大鼠脑内纹状体多巴胺含量的影响。 方法 建立逆转录病毒介导的BDNF表达质粒，并转染成肌细胞进行PD大鼠脑内纹状体移植。 结果 细胞移植后第2和第8周，移植组毁损侧纹状体多巴胺含量〔分别为（957.53±88.95）和(1 040.29±104.78) pg/mg〕较对照组〔分别为(335.98±102.48)和(327.88±70.23) pg/mg〕明显增加（均为P＜0.01），并可维持2个月之久。 结论 脑源性神经营养因子基因工程成肌细胞脑内纹状体移植，可使脑内纹状体多巴胺含量明显增加，可能为帕金森病的治疗提供了一种新的治疗方法。
The measurement of dopamine content in the striatum of Parkinson's rat model after gene therapy
QU Shen, HE Xiaolong, SHI Jian, et al. Department of Neurobiology, Second Military Medical University, Shanghai 200433
【Abstract】 Objective To observe the role of genetically engineered myoblast on Parkinson's rat models. Methods Genetically modified myoblasts capable of producing brain derived neurotrophic factor (BDNF) were grafted into the striatum of Parkinson's rats and the content of dopamine in the striatum was detected with high-performance liquid chromatography (HPLC) after transplantation at different time. Results In the graft group, the dopamine content in the striatum on the lesioned side increased significantly as compared with that in the control group (957.53 to 335.98 pg/mg in week 2 and 1 040.29 to 327.88 pg/mg in week 8, P＜0.01 in both). Conclusions BDNF secreting myoblasts may promote the survival of neuron and may proivde a new approach to the treatment of Parkinson's disease.
【Key words】 Parkinson disease Brain-derived neurotrophic factor Dopamine Gene therapy
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