Change of the rotational behavior after implantation of microencapsulated PC12 cells in PD rats
Lu Shengqing, Zhang Kecheng, Liu Haipeng, et al.
(Department of Neurosurgery, Xinqiao Hospital, Third Military Medical University, Chongqing 400037)
【Abstract】 Objective To observe the rotational behavior of Parkinson's Disease (PD)rats after implantation of microencapsulated PC12 cells. Methods A new method of immunoisolation (microencapsulation) was utilized for encapsulation of dopamine-secreting PC12 cells, and the cells were implanted into the brains of Parkinson’s Disease rats. And then, the change of apomorphine-induced rotational behavior of the PD rats was observed. Tyrosine hydroxylase (TH)antibody immunohistochemistry stains of the cells in the implanted area were also proceeded. Results The rotational behavior of PD rats reduced significantly. The microcapsules in the PD rats’ brains were intact and TH immunopositive PC12 cells could exist for 3 months postimplantation. Conclusion The rotational behavior of PD rats can be promoted by implantation of microencapsulated PC12 cells.
【Key words】 Microencapsulation PC12 cell Implantation Parkinson’s Disease Rotational behavior
材料与方法
